What question did this study set out to answer?

To determine the therapeutic impact and critical time windows of interferon-beta, its inducer pI:pC, and anti-interferon-beta antibodies on Coxsackievirus B3-induced myocarditis.

January 1, 1985Journal of Interferon Research

Ameliorating Effect of IFN-β and Anti-IFN-β on Coxsackievirus B3-Induced Myocarditis in Mice

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Why the study?

Does administration of IFN-beta, pI:pC, or anti-IFN-beta alter myocardial lesions and virus titers in CVB3-induced myocarditis in mice?

Population

Adolescent CD-1 mice inoculated with coxsackievirus B3 and in vitro primary cultures of murine neonatal skin…

Design

Preclinical

Follow-up

7 days postinoculation

Authors

CLC. W. LuttonThe University of Texas Health Science Center at San Antonio CGCharles J. GaunttThe University of Texas Health Science Center at San Antonio

Discussion

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Implication

No clinical translation yet for viral myocarditis; leaves open time-dependent IFN-beta effects in CVB3 models.

Key Points

To determine the therapeutic impact and critical time windows of interferon-beta, its inducer pI:pC, and anti-interferon-beta antibodies on Coxsackievirus B3-induced myocarditis.
Inoculated adolescent CD-1 mice with Coxsackievirus B3 (CVB3) and administered murine IFN-β, pI:pC, or anti-murine IFN-β antiserum at -24, 0, 24, or 72 hours postinoculation.
Assessed cardiac lesion counts, quantified heart tissue viral titers at 3 and 7 days postinoculation, and evaluated viral replication in primary murine neonatal skin fibroblasts in vitro.
Administration of IFN-β or pI:pC at -24, 0, or 24 hours postinoculation significantly reduced myocardial lesions, whereas administration at 72 hours provided no protective effect.
Inoculation of anti-murine IFN-β antiserum at 72 hours postinoculation significantly reduced myocardial lesion counts, indicating a harmful role for late-stage endogenous interferon.
Inoculation of pI:pC at -24, 0, or 24 hours significantly reduced heart virus titers, while direct IFN-β did not alter heart viral titers at 3 or 7 days despite demonstrating antiviral activity in vitro.

Structured PICO

Does administration of IFN-beta, pI:pC, or anti-IFN-beta alter myocardial lesions and virus titers in CVB3-induced myocarditis in mice?

Population

Adolescent CD-1 mice inoculated with coxsackievirus B3 (CVB3) and in vitro primary cultures of murine neonatal skin fibroblasts

Intervention

Murine interferon beta (IFN-beta) or polyriboinosinic: polyribocytidylic acid copolymer (pI:pC) at -24, 0, 24, or 72 h postinoculation, or anti-murine IFN-beta at 72 h postinoculation

Outcome

Number of virus-induced myocardial lesions and virus titers in heart tissuessurrogate

Early administration of interferon is beneficial in CVB3-induced myocarditis, whereas its absence later (72 h post-infection) appears beneficial, suggesting a time-dependent role of interferon in viral myocarditis.

Cite This Study

Lutton et al. (1985) studied this question.

synapsesocial.com/papers/6a912e718790b994430a24df https://doi.org/10.1089/jir.1985.5.137

Topics

Myocarditis

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Ameliorating Effect of IFN-β and Anti-IFN-β on Coxsackievirus B3-Induced Myocarditis in Mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Ameliorating Effect of IFN-β and Anti-IFN-β on Coxsackievirus B3-Induced Myocarditis in Mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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