What question did this study set out to answer?

To determine whether immortalized rat Schwann cell lines can express myelin-associated glycoprotein in culture without neuronal stimulation.

April 1, 1991Journal of Neurochemistry

Expression of the Myelin‐Associated Glycoprotein in Cultures of Immortalized Schwann Cells

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Authors

SGShuichiro GodaSoka University JHJeffrey A. HammerNorthwestern University DKDavid KobilerIsrael Institute for Biological Research

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Implication

In vitro study demonstrates high myelin-associated glycoprotein expression in immortalized rat Schwann cells, indicating a viable culture model for studying peripheral nerve myelination.

Key Points

To determine whether immortalized rat Schwann cell lines can express myelin-associated glycoprotein in culture without neuronal stimulation.
Generated and evaluated immortalized rat Schwann cell lines through repetitive passaging, focusing on the S-16 line.
Quantified protein levels by radioimmunoassay and determined membrane localization using trypsin digestion, neuraminidase treatment, and periodate-mediated [3H]borohydride surface radiolabeling.
The S-16 cell line expressed myelin-associated glycoprotein at approximately 1 ng/µg of total protein, matching levels found in adult sciatic nerve.
The synthesized protein predominantly comprised the shorter isoform (S-MAG) with in vivo-like posttranslational glycosylation, sulfation, and phosphorylation, localized primarily to the cell surface.
S-16 cells stained positive for galactocerebroside but demonstrated little to no detectable P0 glycoprotein or myelin basic protein.

Cite This Study

Goda et al. (1991) studied this question.

synapsesocial.com/papers/6a9132b5d613fa9f3b4658a2 https://doi.org/10.1111/j.1471-4159.1991.tb11432.x

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Implication

In vitro study demonstrates high myelin-associated glycoprotein expression in immortalized rat Schwann cells, indicating a viable culture model for studying peripheral nerve myelination.

Expression of the Myelin‐Associated Glycoprotein in Cultures of Immortalized Schwann Cells

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Implication

Key Points

Cite This Study

Expression of the Myelin‐Associated Glycoprotein in Cultures of Immortalized Schwann Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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