What question did this study set out to answer?

To evaluate whether a codon-optimized, viral 2A-linked bacterial lux operon can efficiently generate autonomous bioluminescence without external chemical substrates across diverse human cell types.

May 2, 2014PLoS ONEOpen Access

Expression of a Humanized Viral 2A-Mediated lux Operon Efficiently Generates Autonomous Bioluminescence in Human Cells

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Authors

TXTingting XuChongqing University of Posts and Telecommunications SRSteven RippUniversity of Tennessee at Knoxville GSGary S. SaylerUniversity of Tennessee at Knoxville

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Implication

In vitro study demonstrates autonomous bioluminescence across multiple human cell lines via a viral 2A-linked lux operon, indicating potential for continuous automated bioreporting.

Key Points

To evaluate whether a codon-optimized, viral 2A-linked bacterial lux operon can efficiently generate autonomous bioluminescence without external chemical substrates across diverse human cell types.
Codon-optimized the bacterial lux cassette and assembled it into a single synthetic human expression operon linked by viral 2A peptide elements.
Transiently and stably transfected human embryonic kidney, breast cancer, and colorectal cancer cell lines with the humanized operon.
Assessed cytotoxicity and validated bioreporter efficacy through continuous population tracking, a modified E-SCREEN assay for estrogen detection, and a Zeocin-based cytotoxicity assay.
Generated autonomous bioluminescence in all tested human cell lines without the induction of measurable cytotoxicity.
Achieved detection limits and EC50 values comparable to traditional substrate-dependent reporter systems during chemical and hormonal tracking assays.

Cite This Study

Xu et al. (2014) studied this question.

synapsesocial.com/papers/6a91363553e347e21eb3924f https://doi.org/10.1371/journal.pone.0096347

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Implication

In vitro study demonstrates autonomous bioluminescence across multiple human cell lines via a viral 2A-linked lux operon, indicating potential for continuous automated bioreporting.

Expression of a Humanized Viral 2A-Mediated lux Operon Efficiently Generates Autonomous Bioluminescence in Human Cells

Discussion

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Implication

Key Points

Cite This Study

Expression of a Humanized Viral 2A-Mediated lux Operon Efficiently Generates Autonomous Bioluminescence in Human Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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