What does this research mean for the field?

High-resolution proton-detected NMR spectra of proteins can be successfully obtained under cryogenic dynamic nuclear polarization (DNP) conditions by utilizing extensive deuteration, fast magic-angle spinning, and high magnetic fields. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.CHALLENGES_CONSENSUS.

What question did this study set out to answer?

To determine whether high-resolution, proton-detected solid-state NMR spectra of biomolecules can be achieved under cryogenic dynamic nuclear polarization conditions.

August 28, 2026Angewandte ChemieOpen Access

Preserving Proton Resolution Under Cryogenic Dynamic Nuclear Polarization for Biomolecular Solid‐State NMR

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To determine whether high-resolution, proton-detected solid-state NMR spectra of biomolecules can be achieved under cryogenic dynamic nuclear polarization conditions.
Coupled cryogenic dynamic nuclear polarization with fast magic-angle spinning and high magnetic fields.
Applied extensive deuteration and selective 13CHD2 methyl labeling to microcrystalline proteins and amyloid fibrils.
Performed quantitative linewidth analysis and proton-detected 1H-1H correlation spectroscopy.
Site-resolved proton detection was successfully preserved in both microcrystalline protein samples and amyloid fibrils under cryogenic conditions.
Proton coherence lifetimes remained long because fast spinning, strong magnetic fields, and proton dilution suppressed homogeneous broadening, leaving linewidths dominated by refocusable inhomogeneous broadening.
High sensitivity permitted structurally informative proton-detected 1H-1H correlation spectroscopy on challenging assemblies.

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Implication

Spectroscopic study demonstrates site-resolved proton detection in microcrystalline proteins and amyloid fibrils, indicating a feasible high-sensitivity approach for biomolecular NMR.

Cite This Study

Tolchard et al. (2026) studied this question.

synapsesocial.com/papers/6a91466cd15324a1df3aa0cf https://doi.org/10.1002/ange.3761769

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Implication

Spectroscopic study demonstrates site-resolved proton detection in microcrystalline proteins and amyloid fibrils, indicating a feasible high-sensitivity approach for biomolecular NMR.

Preserving Proton Resolution Under Cryogenic Dynamic Nuclear Polarization for Biomolecular Solid‐State NMR

Key Points

Discussion

Implication

Cite This Study

Preserving Proton Resolution Under Cryogenic Dynamic Nuclear Polarization for Biomolecular Solid‐State NMR

Key Points

Discussion

Implication

Cite This Study

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