What question did this study set out to answer?

To determine how axonal growth cones detect and integrate discontinuous, substrate-bound ephrinA5 gradients during topographic map formation.

June 8, 2006Development

Growth cone navigation in substrate-bound ephrin gradients

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Authors

APAnne C. von PhilipsbornUniversity of Fribourg SLSusanne LangUniversity of Cambridge JLJürgen Loeschinger

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Implication

In vitro study reveals that chick retinal axon growth cones arrest along discontinuous ephrinA5 gradients via local and cumulative cues, indicating dual-parameter control in topographic mapping.

Key Points

To determine how axonal growth cones detect and integrate discontinuous, substrate-bound ephrinA5 gradients during topographic map formation.
Generated discontinuous gradients of substrate-bound ephrinA5 using microcontact printing to produce submicron-sized protein spots with varying dimensions and spatial separations.
Cultured chick temporal retinal axons on the patterned substrates and quantitatively analyzed growth cone navigation, filopodial activity, and axon outgrowth.
Growth cones integrated discontinuous ephrin distributions and arrested at distinct stop zones while maintaining active filopodial exploration.
The spatial position of axon termination depended directly on both the steepness of the gradient and the surface density of bound ephrinA5.
Axon arrest was governed by a combined readout of local ligand concentration and total encountered ephrin rather than either metric independently.

Cite This Study

Philipsborn et al. (2006) studied this question.

synapsesocial.com/papers/6a9150804f46675386afa68a https://doi.org/10.1242/dev.02412

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Implication

In vitro study reveals that chick retinal axon growth cones arrest along discontinuous ephrinA5 gradients via local and cumulative cues, indicating dual-parameter control in topographic mapping.

Growth cone navigation in substrate-bound ephrin gradients

Discussion

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Implication

Key Points

Cite This Study

Growth cone navigation in substrate-bound ephrin gradients

Discussion

Member takes

Implication

Key Points

Cite This Study

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