April 25, 1995Proceedings of the National Academy of SciencesOpen Access

Rescue, propagation, and partial purification of a helper virus-dependent adenovirus vector.

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Population

293 cell line (human embryonic kidney) and COS-7 monkey kidney cell line

Design

Preclinical

Authors

KMK MitaniSaitama Medical University FGFrank L. GrahamOntario Institute for Cancer Research CCC. Thomas CaskeyBaylor College of Medicine

Discussion

Member takes

Implication

Enables scalable helper-dependent adenoviral vector production; leaves open clinical translation and safety in humans.

Structured PICO

Population

293 cell line (human embryonic kidney) and COS-7 monkey kidney cell line

Intervention

Defective human adenovirus serotype 5 DNA (pKM74) co-introduced with wild-type adenovirus serotype 2 (Ad2) helper

Outcome

Titer of recombinant virus and separation from helper virus

A helper-dependent adenoviral vector system was successfully rescued and propagated, offering a potential alternative to current adenoviral vectors by eliminating viral protein expression.

Limitations

Recombination between vector and helper virus (deletion of pBR sequence)
Current system is not directly applicable for human gene therapy

Cite This Study

Mitani et al. (1995) studied this question.

synapsesocial.com/papers/6a915462d613fa9f3b465f31 https://doi.org/10.1073/pnas.92.9.3854

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Rescue, propagation, and partial purification of a helper virus-dependent adenovirus vector.

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Rescue, propagation, and partial purification of a helper virus-dependent adenovirus vector.

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider