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A sensitive and reproducible HPLC method utilizing a commercially available chiral α1‐acid glycoprotein (AGP) phase has been developed to separate and quantify the enantiomers of nicotine. The method is suitable for routine use as indicated by column life. The quantification of (R/S:0.05/99.95)‐nicotine or (R/S:99/1)‐nicotine was possible. In addition, the separation or at least partial separation of the enantiomers of nornicotine and nornicotine‐derived compounds was achieved.
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Demetriou et al. (1993) studied this question.
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