Analysis of genetic markers in new breeding stocks of the yeast Saccharomycopsis lipolytica

For development of breeding stocks of S. lipolytica for genetic investigations we isolated and identified several auxotrophic mutants. After selection of strains with normal meiotic segregation pattern for the markers metA, lysA, argA, leuA, uraA, hisA it was shown that the loci metA and lysA and the loci argA and leuA are linked, crossing over frequencies are 25 ± 0.9% and 30 ± 1.2%, respectively. We identified several complementation groups. The marker uraA is allel to ura3 from strains of the Donner Laboratory (USA). These results are the basis for selection of special mutants for forthcoming investigations. The existence of linkage fragments makes it possible to use these strains as tester strains for genetic analysis.