What question did this study set out to answer?

To isolate and characterize nicotianamine synthase (NAS) genes in maize and determine how their expression, protein localization, and enzymatic activity respond to iron nutritional status.

August 1, 2003PLANT PHYSIOLOGYOpen Access

Three Nicotianamine Synthase Genes Isolated from Maize Are Differentially Regulated by Iron Nutritional Status

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Authors

DMDaichi MizunoThe University of Tokyo KHKyoko HiguchiTokyo University of Agriculture TSTatsuya SakamotoHiroshima University

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Implication

Gene characterization study reveals differential regulation of three nicotianamine synthase genes in maize, suggesting distinct roles in plant iron homeostasis.

Key Points

To isolate and characterize nicotianamine synthase (NAS) genes in maize and determine how their expression, protein localization, and enzymatic activity respond to iron nutritional status.
Isolated one genomic clone (ZmNAS3) and two cDNA clones (ZmNAS1 and ZmNAS2) from maize (Zea mays cv Alice).
Assessed gene expression under iron-deficient and iron-sufficient growth conditions.
Evaluated subcellular localization using green fluorescent protein (sGFP) fusions in Allium cepa epidermal cells and measured in vitro enzymatic activity.
ZmNAS1 and ZmNAS2 were induced exclusively in iron-deficient roots, whereas ZmNAS3 was expressed under iron-sufficient conditions and downregulated during iron deficiency.
ZmNAS1-sGFP and ZmNAS2-sGFP localized to cytoplasmic spots, whereas ZmNAS3-sGFP was uniformly distributed throughout the cytoplasm in onion epidermal cells.
In vitro NAS activity was confirmed for ZmNAS1 and ZmNAS3, but absent in ZmNAS2, which possessed a duplicated structure yielding a larger molecular mass (65.8 kD).

Cite This Study

Mizuno et al. (2003) studied this question.

synapsesocial.com/papers/6a915c75fe6bc987f0fb58fd https://doi.org/10.1104/pp.102.019869

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Implication

Gene characterization study reveals differential regulation of three nicotianamine synthase genes in maize, suggesting distinct roles in plant iron homeostasis.

Three Nicotianamine Synthase Genes Isolated from Maize Are Differentially Regulated by Iron Nutritional Status

Discussion

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Implication

Key Points

Cite This Study

Three Nicotianamine Synthase Genes Isolated from Maize Are Differentially Regulated by Iron Nutritional Status

Discussion

Member takes

Implication

Key Points

Cite This Study

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