What question did this study set out to answer?

To examine how reversible interactions between a single macromolecular protein species and constituents of a buffer solution can produce multiple electrophoretic zones.

March 1, 1966Biochemistry

Multiple Electrophoretic Zones Arising from Protein-Buffer Interaction*

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Authors

JCJohn CannGates (United States)

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Implication

Biochemical analysis demonstrates multiple electrophoretic zones arising from protein-buffer interactions, indicating that multiple peaks do not always imply sample heterogeneity.

Key Points

To examine how reversible interactions between a single macromolecular protein species and constituents of a buffer solution can produce multiple electrophoretic zones.
Analyzed the transport mechanics and boundary patterns of proteins undergoing reversible complexation with buffer constituents during electrophoresis.
Evaluated the conditions under which chemical equilibrium and migration velocities yield multiple distinct peaks.
Demonstrated that reversible association between a single homogeneous protein and buffer components can resolve into multiple distinct electrophoretic zones.
Showed that multimodal electrophoretic boundaries do not necessarily reflect macromolecular heterogeneity in a biological sample.

Cite This Study

John Cann (1966) studied this question.

synapsesocial.com/papers/6a9172faddfd49c27e4639ba https://doi.org/10.1021/bi00867a042

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Implication

Biochemical analysis demonstrates multiple electrophoretic zones arising from protein-buffer interactions, indicating that multiple peaks do not always imply sample heterogeneity.

Multiple Electrophoretic Zones Arising from Protein-Buffer Interaction*

Discussion

Member takes

Implication

Key Points

Cite This Study

Multiple Electrophoretic Zones Arising from Protein-Buffer Interaction*

Discussion

Member takes

Implication

Key Points

Cite This Study

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Also Consider