What question did this study set out to answer?

To characterize the distribution, electrophysiological actions, and intracellular signaling cascades of bombesin-related peptide receptors in the central nervous system.

June 1, 1996Annals of the New York Academy of Sciences

Bombesin Receptors in the Brain

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To characterize the distribution, electrophysiological actions, and intracellular signaling cascades of bombesin-related peptide receptors in the central nervous system.
Evaluated electrophysiological responses to bombesin-related peptides in rat brain slices containing dorsal raphe neurons and the suprachiasmatic nucleus.
Expressed cloned human brain bombesin receptors in Chinese hamster ovary cells and Xenopus oocytes to profile pharmacological properties and second-messenger cascades.
Neuromedin B receptors depolarize dorsal raphe neurons via reduced potassium conductance, while gastrin-releasing peptide receptors excite suprachiasmatic nucleus neurons through potassium conductance closure and opening of an unidentified cation conductance.
Activation of bombesin receptors in Chinese hamster ovary cells and Xenopus oocytes stimulates phosphatidylinositol 4,5-bisphosphate hydrolysis to generate inositol trisphosphate and mobilize intracellular calcium.
Downstream signaling in expression systems culminates in calcium-activated chloride conductance rather than modulating potassium conductance, demonstrating divergence from native neuronal pathways.

Authors

GWG.N. WoodruffAstraZeneca (United Kingdom)MHMatthew D. HallNational Center for Advancing Translational Sciences TRTracy ReynoldsNovartis (Switzerland)

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Implication

Laboratory investigation demonstrates bombesin receptor-mediated neuronal excitation in serotonergic circuits, highlighting mechanistic differences between native neurons and expression systems.

Cite This Study

Woodruff et al. (1996) studied this question.

synapsesocial.com/papers/6a917ec8bd296d8bc5121182 https://doi.org/10.1111/j.1749-6632.1996.tb15126.x

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Implication

Laboratory investigation demonstrates bombesin receptor-mediated neuronal excitation in serotonergic circuits, highlighting mechanistic differences between native neurons and expression systems.

Bombesin Receptors in the Brain

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Bombesin Receptors in the Brain

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