We describe buffer for both immunoelectrophoresis and zone electrophoresis procedures in agarose gels, Tricine [N-tris(hydroxymethyl)methylglycine] and Tris [2-amino-2-(hydroxymethyl)-1,3-propanediol] being the main components. This buffer eliminates the procurement cost and inventory control associated with formulation of barbital buffers. We compare it with other common buffer formulations.
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Monthony et al. (1978) studied this question.