N -phenyl- N′ 1,2,3-thiadiazol-5-ylurea (thidiazuron) and several substituted pyridyl phenylurea compounds have been demonstrated to stimulate in vitro meristem and shoot formation at unusually low concentrations. These compounds appear to have strong cytokinin-like effects on a wide range of species and on species that respond little to conventional cytokinins. Thidiazuron has been reported to stimulate shoot proliferation in several woody species (e.g., Acer and Malus ). The addition of 0.5μ m N -(2-chloro-4-pyridyl)- N′ -phenylurea (CPPU) to the culture medium caused dramatic shoot number increases in hardy dedicuous azaleas ( Rhododendron sp.) cultured in vitro. In petunia ( Petunia × hybrida Hort. Vilm.-Andr.) leaf test systems both thidiazuron and CPPU caused greater proliferation when used as explant dips or in the medium than similar treatments with N -(phenylmethyl)-1 H -purin-6-amine (BA). Further possible applications and roles for these compounds are discussed.
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Fellman et al. (1987) studied this question.