What question did this study set out to answer?

Identify the molecular polymorphism underlying the clinically important human platelet alloantigen system Bra/Brb on glycoprotein Ia.

November 1, 1993Journal of Clinical InvestigationOpen Access

The human platelet alloantigens Br(a) and Brb are associated with a single amino acid polymorphism on glycoprotein Ia (integrin subunit alpha 2).

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Authors

SSS SantosoJustus-Liebig-Universität Gießen RKR KalbSGS Institut Fresenius (Germany)MWMatthias M. WalkaHumboldt-Universität zu Berlin

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Implication

Genetic analysis reveals a single amino acid polymorphism on glycoprotein Ia defines Bra and Brb alloantigens in human platelets, indicating opportunities for prenatal diagnosis.

Key Points

Identify the molecular polymorphism underlying the clinically important human platelet alloantigen system Bra/Brb on glycoprotein Ia.
Amplified platelet GPIa mRNA transcripts via PCR and sequenced cDNA derived from Br(a/a) and Brb/b individuals.
Confirmed phenotypic segregation using MnlI restriction fragment length polymorphism (RFLP) analysis across serologically characterized subjects.
Evaluated platelet adhesion to collagen types I, III, and V in homozygous platelets and in the presence of anti-Bra or anti-Brb alloantibodies.
Identified an A-to-G nucleotide substitution at base 1648 of GPIa cDNA, replacing lysine (AAG) in Bra with glutamic acid (GAG) in Brb at amino acid residue 505.
Observed no differences in adhesion to collagen types I, III, or V between Bra and Brb homozygous platelets, and alloantibodies against either antigen did not impair collagen binding.

Cite This Study

Santoso et al. (1993) studied this question.

synapsesocial.com/papers/6a918aaaeb80182e01121b0a https://doi.org/10.1172/jci116849

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Implication

Genetic analysis reveals a single amino acid polymorphism on glycoprotein Ia defines Bra and Brb alloantigens in human platelets, indicating opportunities for prenatal diagnosis.

The human platelet alloantigens Br(a) and Brb are associated with a single amino acid polymorphism on glycoprotein Ia (integrin subunit alpha 2).

Discussion

Member takes

Implication

Key Points

Cite This Study

The human platelet alloantigens Br(a) and Brb are associated with a single amino acid polymorphism on glycoprotein Ia (integrin subunit alpha 2).

Discussion

Member takes

Implication

Key Points

Cite This Study

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