Nucleotides such as DNA, polyribonucleotides, and NAD+ suppress the immunoglobulin production stimulating activity of GAPDH in vitro.
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Nucleotide suppression of GAPDH IPSF warrants in vitro assay caution; leaves open any in vivo or clinical immunomodulatory relevance.
We have previously identified glyceraldehyde-3-phosphate dehydrogenase as an immunoglobulin production stimulating factor (IPSF) which facilitated immunoglobulin production by hybridomas and lymphocytes. The IPSF activity of this enzyme was suppressed by the coexistence of some sorts of nucleotides. We now report that the IPSF effect of GAPDH was suppressed by the coexistence of DNA, the inhibiting effect of degraded DNA being inferior to that of long-chain DNA. Both single-stranded and double-stranded synthetic polyribonucleotides also inhibited the IPSF activity of GAPDH. Moreover, nicotinamide adenine dinucleotide (NAD+) repressed the IPSF effect.
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Sugahara et al. (1998) studied this question.
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