July 15, 2000Journal of VirologyOpen Access

Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function

View Full Paper

Ask AI

Bookmark

Population

BSC-1, FRhK-4, and Huh-7 cells

Comparison

Transfection with a plasmid containing a… vs Transfection with a control, null-expression…

Design

Preclinical

Authors

Discussion

Cell-type specificity in GAPDH-HAV effects warrants human hepatocyte validation; leaves open antiviral targeting.

Population

BSC-1, FRhK-4, and Huh-7 cells

Intervention

Transfection with a plasmid containing a dicistronic transcriptional unit resulting in GAPDH overexpression

Comparator

Transfection with a control, null-expression plasmid identical except for a frameshift mutation within the 5' GAPDH coding sequence

Outcome

Renilla luciferase (RLuc) production as a measure of IRES activitysurrogate

Binding of GAPDH to the IRES of Hepatitis A virus suppresses cap-independent viral translation in vivo in African green monkey kidney cells.

Yi et al. (2000) studied this question.

View Full Paper

Ask AI

Bookmark