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August 1, 1994Journal of VirologyOpen Access

Low efficiency of the 5' nontranslated region of hepatitis A virus RNA in directing cap-independent translation in permissive monkey kidney cells

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Population

HAV-permissive monkey kidney cell line (BT7-H) stably expressing T7 RNA polymerase

Comparison

Transfection with DNA containing T7 promoter and… vs Transcripts containing EMC virus IRES or short…

Design

Preclinical

Authors

LWLinda WhetterUniversity of North Carolina at Chapel HillSDStephen DayGeorgetown UniversityOEOrna Elroy‐SteinTel Aviv University

Discussion

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Overview

HAV IRES inefficiency in kidney cells leaves open its mechanism and factor requirements in hepatocytes; hypothesis-generating for picornavirus translation.

Structured PICO

P
Population
HAV-permissive monkey kidney cell line (BT7-H) stably expressing T7 RNA polymerase
I
Intervention
Transfection with DNA containing T7 promoter and HAV 5'NTR (bicistronic and monocistronic transcripts, various deletions)
C
Comparator
Transcripts containing EMC virus IRES or short nonpicornavirus 5' nontranslated leader sequence
O
Outcome
Translation efficiency of downstream reporter protein (bacterial chloramphenicol acetyltransferase)surrogate

The HAV IRES directs translation inefficiently in monkey kidney cells and may have a unique requirement for intact p220 or other limiting cellular translation factors.

Cite This Study

Whetter et al. (1994) studied this question.

synapsesocial.com/papers/6a8212646cfba4e31a9fd867https://doi.org/10.1128/jvi.68.8.5253-5263.1994
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5' sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system.1989 · 405 citations
  2. 2Poliovirus proteinase 2A induces cleavage of eucaryotic initiation factor 4F polypeptide p2201987 · 322 citations
  3. 3In vitro characterization of an internal ribosomal entry site (IRES) present within the 5' nontranslated region of hepatitis A virus RNA: comparison with the IRES of encephalomyocarditis virus1994 · 160 citations
  4. 4The 5' nontranslated region of hepatitis A virus RNA: secondary structure and elements required for translation in vitro1991 · 211 citations
  5. 5Translational enhancement of the poliovirus 5' noncoding region mediated by virus-encoded polypeptide 2A.1992 · 138 citations