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February 1, 1994Journal of VirologyOpen Access

In vitro characterization of an internal ribosomal entry site (IRES) present within the 5' nontranslated region of hepatitis A virus RNA: comparison with the IRES of encephalomyocarditis virus

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Population

Rabbit reticulocyte lysates translating synthetic bicistronic RNAs containing hepatitis A virus 5'…

Comparison

HAV 5'NTR constructs vs Encephalomyocarditis virus (EMCV) IRES constructs

Design

Preclinical

Authors

EBEdwin A. BrownUniversity of North Carolina at Chapel HillAZAllan ZajacUniversity of Alabama at BirminghamSLStanley M. LemonUniversity of North Carolina at Chapel Hill

Discussion

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Overview

HAV IRES supports weak internal initiation; leaves open its role in viral translation efficiency pending mammalian validation.

Structured PICO

P
Population
Rabbit reticulocyte lysates translating synthetic bicistronic RNAs containing hepatitis A virus (HAV) 5' nontranslated region (5'NTR)
I
Intervention
HAV 5'NTR constructs (including deletion mutants and 5' cap additions)
C
Comparator
Encephalomyocarditis virus (EMCV) IRES constructs
O
Outcome
Translation initiation efficiencysurrogate

The HAV 5'NTR contains an internal ribosome entry site (IRES) that is significantly less active than the EMCV IRES, likely due to reduced affinity for limiting cellular translation factors.

Cite This Study

Brown et al. (1994) studied this question.

synapsesocial.com/papers/6a7d24482935e92dbc77585fhttps://doi.org/10.1128/jvi.68.2.1066-1074.1994
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Complete nucleotide sequence of wild-type hepatitis A virus: comparison with different strains of hepatitis A virus and other picornaviruses1987 · 385 citations
  2. 2Low efficiency of the 5' nontranslated region of hepatitis A virus RNA in directing cap-independent translation in permissive monkey kidney cells1994 · 119 citations
  3. 3The 5' nontranslated region of hepatitis A virus RNA: secondary structure and elements required for translation in vitro1991 · 211 citations
  4. 4Conserved tertiary structural elements in the 5’ nontranslated region of cardiovirus, aphthovirus and hepatitis A virus RNAs1993 · 57 citations
  5. 5Sequence and structural elements that contribute to efficient encephalomyocarditis virus RNA translation1992 · 235 citations