September 1, 2001Journal of VirologyOpen Access

Activity of the Hepatitis A Virus IRES Requires Association between the Cap-Binding Translation Initiation Factor (eIF4E) and eIF4G

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Population

in vitro translation in standard and eIF4G-depleted rabbit reticulocyte lysates

Comparison

Cleavage of eIF4G by foot-and-mouth disease… vs Standard conditions / untreated

Design

Preclinical

Authors

Discussion

May guide future antiviral strategies; leaves open confirmation in human cells and broader viral applicability.

Population

in vitro translation in standard and eIF4G-depleted rabbit reticulocyte lysates

Intervention

Cleavage of eIF4G by foot-and-mouth disease L-proteases, addition of eIF4E-binding protein 1, or addition of m(7)GpppG cap analogue

Comparator

Standard conditions / untreated

Outcome

Translation initiation dependent on the IRES of hepatitis A virus (HAV)

Initiation on the HAV IRES requires that eIF4E be associated with eIF4G and that the cap-binding pocket of eIF4E be empty and unoccupied.

Ali et al. (2001) studied this question.

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