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January 1, 1995Nucleic Acids ResearchOpen Access

Picornavirus internal ribosome entry segments: comparison of translation efficiency and the requirements for optimal internal initiation of translationin vitro

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Population

in vitro translation system using a single dicistronic mRNA

Comparison

Six different picornaviral IRESes and hepatitis… vs Comparison among the different IRESes

Design

Preclinical

Authors

ABAndrew M. BormanBristol-Myers Squibb (Germany)JBJean‐Luc BaillyCentre National de la Recherche ScientifiqueMGMarc GirardUniversity of Chieti-Pescara

Discussion

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Implication

May guide IRES selection for experimental cardiac vectors; leaves open in vivo translation.

Structured PICO

P
Population
in vitro translation system using a single dicistronic mRNA
I
Intervention
Six different picornaviral IRESes and hepatitis C virus IRES under various parameters (salt concentration, HeLa cell proteins, foot-and-mouth disease virus Lb or human rhinovirus 2A proteinase)
C
Comparator
Comparison among the different IRESes
O
Outcome
Ability to direct internal initiation of translationsurrogate

Picornaviral IRESes exhibit dramatically different functional requirements for efficient internal ribosome entry in vitro, with cardio- and aphthovirus IRESes showing the highest translation efficiency.

Cite This Study

Borman et al. (1995) studied this question.

synapsesocial.com/papers/6a7ff511a89a13a921d475bchttps://doi.org/10.1093/nar/23.18.3656
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1In vitro characterization of an internal ribosomal entry site (IRES) present within the 5' nontranslated region of hepatitis A virus RNA: comparison with the IRES of encephalomyocarditis virus1994 · 160 citations
  2. 2Low efficiency of the 5' nontranslated region of hepatitis A virus RNA in directing cap-independent translation in permissive monkey kidney cells1994 · 119 citations
  3. 3Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo1989 · 419 citations
  4. 4Purification of two picornaviral 2A proteinases: Interaction with eIF-4.gamma. and influence on the in vitro translation1993 · 181 citations
  5. 5Differences in replication of attenuated and neurovirulent polioviruses in human neuroblastoma cell line SH-SY5Y1989 · 170 citations