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March 1, 1997Nucleic Acids ResearchOpen Access

Comparison of Picornaviral IRES-Driven Internal Initiation of Translation in Cultured Cells of Different Origins

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Population

Cultured cell lines infected with recombinant vaccinia virus expressing T7 polymerase and transfected with…

Comparison

Six picornaviral internal ribosome entry… vs Parental construct pXLJO and comparison among…

Design

Preclinical

Authors

ABAndrew M. BormanBristol-Myers Squibb (Germany)PMPhilippe Le MercierEuropean Bioinformatics InstituteMGMarc GirardUniversity of Chieti-Pescara

Discussion

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Overview

Cell-specific IRES patterns and 2A rescue are hypothesis-generating for viral tropism; leaves open relevance to human disease models.

Structured PICO

P
Population
Cultured cell lines (Neuro-2A, SKNBE, HeLa, HepG2, FRhK4, L929, BHK21, HeLa S3) infected with recombinant vaccinia virus expressing T7 polymerase and transfected with dicistronic plasmids
I
Intervention
Six picornaviral internal ribosome entry segments (IRESes) (poliovirus type 1, ECHO virus type 25, human rhinovirus type 2, hepatitis A virus, encephalomyocarditis virus, foot-and-mouth disease virus) and hepatitis C virus IRES
C
Comparator
Parental construct pXLJO and comparison among the different IRESes
O
Outcome
Efficiency of IRES-driven internal initiation of translation (measured as the amount of NS product relative to cyclin B2)surrogate

Picornaviral IRES efficiency varies significantly across different cell types, but viral proteins like the poliovirus 2A proteinase can overcome cell-specific translational defects.

Cite This Study

Borman et al. (1997) studied this question.

synapsesocial.com/papers/6a82136a71d4e96905f1d08ehttps://doi.org/10.1093/nar/25.5.925
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1In vitro translation of poliovirus RNA: utilization of internal initiation sites in reticulocyte lysate1984 · 235 citations
  2. 2The involvement of a spliceosome component in internal initiation of human rhinovirus RNA translation1993 · 137 citations
  3. 3Proteolytic cleavage of initiation factor elF-4γ in the reticulocyte lysate inhibits translation of capped mRNAs but enhances that of uncapped mRNAs1995 · 93 citations
  4. 4Purification of two picornaviral 2A proteinases: Interaction with eIF-4.gamma. and influence on the in vitro translation1993 · 181 citations
  5. 5Attenuation stem-loop lesions in the 5' noncoding region of poliovirus RNA: neuronal cell-specific translation defects1996 · 72 citations