Key result
Long-range RNA-RNA interaction between nucleotides 24-38 of the 5'NTR and 428-442 of the core-coding sequence of hepatitis C virus down-regulates cap-independent translation via HCV IRES.
Population
Rabbit reticulocyte lysate and HepG2 cells (in vitro models of Hepatitis C virus translation)
Comparison
Inclusion of the core-coding sequence and… vs Constructs without the core-coding sequence or…
Design
Preclinical
Authors
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Suggests HCV regulatory target for antivirals; leaves open in vivo validation and clinical relevance.
Long-range RNA-RNA interaction between the HCV 5'NTR and the core-coding sequence down-regulates cap-independent translation, suggesting a mechanism for viral gene expression regulation.
Kim et al. (2003) studied Hepatitis C virus (HCV) infection (in vitro model). Site-directed mutations in HCV RNA vs. Wild-type HCV RNA was evaluated on Translational efficiency (IRES-dependent translation). Long-range RNA-RNA interaction between nucleotides 24-38 of the 5'NTR and 428-442 of the core-coding sequence of hepatitis C virus down-regulates cap-independent translation via HCV IRES.
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