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May 1, 1998Journal of VirologyOpen Access

Coding Sequences Enhance Internal Initiation of Translation by Hepatitis A Virus RNA In Vitro

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Population

HeLa cell extracts in vitro

Comparison

Chimeric RNA constructs fusing the 5' noncoding… vs Chimeric constructs fused precisely at the…

Design

Preclinical

Authors

JGJudith GraffInstitute of Medical Microbiology and HygieneEEEllie EhrenfeldNational Institutes of Health

Discussion

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Implication

May inform picornavirus IRES studies; leaves open in vivo relevance and therapeutic applications.

Structured PICO

P
Population
HeLa cell extracts in vitro
I
Intervention
Chimeric RNA constructs fusing the 5' noncoding region of Hepatitis A virus (HAV) to poliovirus (PV) coding sequences, including 114 or more nucleotides from the HAV capsid coding sequences downstream of the initiation codon
C
Comparator
Chimeric constructs fused precisely at the initiation codon (lacking downstream HAV sequences) or constructs with intact HAV 5'-terminal end
O
Outcome
Translation efficiency in vitrosurrogate

HAV coding sequences downstream of the initiation codon are required for efficient internal initiation of translation in vitro.

Cite This Study

Graff et al. (1998) studied this question.

synapsesocial.com/papers/6a891e30cb3766d3cd130c68https://doi.org/10.1128/jvi.72.5.3571-3577.1998
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Low efficiency of the 5' nontranslated region of hepatitis A virus RNA in directing cap-independent translation in permissive monkey kidney cells1994 · 119 citations
  2. 2The 5'-terminal nucleotides of hepatitis A virus RNA, but not poliovirus RNA, are required for infectivity1991 · 43 citations
  3. 3Translation of Sindbis virus mRNA: effects of sequences downstream of the initiating codon1994 · 115 citations
  4. 4Linker scanning mutagenesis of the internal ribosome entry site of poliovirus RNA1992 · 92 citations
  5. 5Coupled translation and replication of poliovirus RNA in vitro: synthesis of functional 3D polymerase and infectious virus1993 · 103 citations