Key result
A modified real-time RT-PCR assay successfully detected nine circulating rotavirus genotypes with a limit of detection of approximately 44 genome copies per reaction and no cross-reactivity.
Population
103 archived RNAs extracted from stools and genotyped during routine U.S. surveillance, representing rare…
Design
Other
Authors
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May aid rotavirus genotyping in stool diagnostics; leaves open prospective validation before routine adoption.
The modified real-time RT-PCR assay provides a sensitive and specific tool for the broad detection of circulating rotavirus genotypes in stool samples.
Freeman et al. (2008) studied Rotavirus (n=103). Modified real-time RT-PCR assay was evaluated on Limit of detection and broad reactivity across rotavirus genotypes. A modified real-time RT-PCR assay successfully detected nine circulating rotavirus genotypes with a limit of detection of approximately 44 genome copies per reaction and no cross-reactivity.
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