Automated Differential Agglutination Technic to Measure Red Cell Survival I. Methodology

Experiments were performed to prove the validity of an automated differential hemagglutination test to measure the survival of transfused red cells. Four antibodies, anti‐A, anti‐B, anti‐CD, and anti‐M, were used to perform differential hemagglutination. This method permitted measurement of the survival of at least two compatible but antigenically identifiable red cell populations in the same recipient. The measurements obtained with the automated method were less variable than those obtained simultaneously with a manual method.