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Polyclonal anti‐deoxynivalenol (vomitoxin) antisera was produced in rabbits. The immunogen was synthesized by using acetyl esterase to deacylate 3‐acetyldeoxynivalenol hemiglutarate and coupling the product to bovine serum albumin using the mixed anhydride reaction. The antiserum was very specific for deoxynivalenol in a microtitration plate ELISA that had a limit of detection of 90 pg per well. A simple, rapid extraction procedure was employed to enable the ELISA to be used for analysis of the deoxynivalenol in wheat.
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Mills et al. (1990) studied this question.
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