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A new procedure utilizing column chromatography has been devised for the purification of yeast glyceraldehyde 3-phosphate dehydrogenase. It yields enzyme of the highest specific activity in a shorter period of time and with a better yield than the traditional procedure. The isozyme isolated is the most abundant and most acidic of the set of isozymes previously identified.
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Stallcup et al. (1972) studied this question.
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