The devised miniculture system provides a comparable alternative to the conventional tube method for assaying neutralizing antibodies against polioviruses.
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Supports miniculture as lab alternative for poliovirus assays; leaves open human validation before clinical use.
1) A miniculture system has been devised using a continuous Vero cell line in the Leibovitz-15 (L-15) medium supplemented with bovine albumin fraction V. A highly humid atmosphere required for the incubation was obtained in a desicator by placing a small vial filled with water.2) The above described system was applied to the titration of human sera for the poliovirus neutralization. A good correlation was obtained between this and the conventional tube method. Further more poliovirus serum neutralization by our system had a tendency of showing a little higher titer in the lower serum dilution area, and a little lower titer in the higher dilution area in comparison to the conventional tube method.
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宗之 et al. (1970) studied this question.
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