Key result
Pretreatment with 200 µg/ml of pomegranate seed oil significantly increased the viability of H9c2 cardiomyocytes exposed to H2O2 from 47% to 88%.
Why the study?
Does pomegranate seed oil protect against H2O2-induced oxidative stress in H9c2 cardiomyocytes?
Does pomegranate seed oil protect against H2O2-induced oxidative stress in H9c2 cardiomyocytes?
Absolute Event Rate: 88% vs 47%
p-value: p=<0.001
Pomegranate seed oil demonstrates protective effects against oxidative stress-induced damage in H9c2 cardiomyocytes in vitro.
Hypothesis-generating for PSO in oxidative cardiac injury; should not yet influence clinical decisions.
OBJECTIVE: )-induced damage in H9c2 cardiomyocytes. MATERIALS AND METHODS: . Cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay. The level of reactive oxygen species (ROS) and lipid peroxidation were measured by fluorimetric methods. RESULTS: significantly decreased cell viability which was accompanied by an increase in ROS production and lipid peroxidation and a decline in superoxide dismutase activity. Pretreatment with PSO increased viability of cardiomyocytes and decrease the elevated ROS production and lipid peroxidation. Also, PSO was able to restore superoxide dismutase activity. CONCLUSION: PSO has protective effect against oxidative stress-induced damage in cardiomyocytes and can be considered as a natural cardioprotective agent to prevent cardiovascular diseases.
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Bihamta et al. (2017) studied H2O2-induced oxidative stress in cardiomyocytes. Pomegranate seed oil (PSO) vs. H2O2 alone was evaluated on Cell viability (% of control) (p=<0.001). Pretreatment with 200 µg/ml of pomegranate seed oil significantly increased the viability of H9c2 cardiomyocytes exposed to H2O2 from 47% to 88%.
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