What question did this study set out to answer?

To thermodynamically and spectroscopically characterize the binding interactions between the minor groove-binding ligand netropsin and synthetic poly d(AT) duplexes.

January 1, 1983ViewOpen Access

Calorimetric and spectroscoptc Investigation of drug - DNA Interactions. I. The binding of netropsin to poly d(AT)

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Authors

LMLuis A. MarkyRutgers, The State University of New Jersey KBKenneth S. BlumenfeldRutgers, The State University of New Jersey KBKenneth J. BreslauerRutgers, The State University of New Jersey

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Implication

Biophysical study reveals exothermic groove binding and substantial thermal stabilization of poly d(AT) by netropsin, suggesting that hydrogen bonding and cation release drive complex formation.

Key Points

To thermodynamically and spectroscopically characterize the binding interactions between the minor groove-binding ligand netropsin and synthetic poly d(AT) duplexes.
Monitored binding using temperature-dependent ultraviolet (UV) absorption and circular dichroism (CD) spectroscopy.
Conducted batch calorimetry and differential scanning calorimetry (DSC) to calculate binding enthalpies and thermal transition behaviors.
Netropsin groove binding produced an exothermic enthalpy of -9.2 kcal/mol of drug bound at 25 °C and -10.7 kcal/mol at 88 °C, accompanied by a positive entropy change.
Duplex saturation raised the thermal melting transition monophasically by 46 °C and decreased the cooperativity of the duplex-to-single-strand transition.

Cite This Study

Marky et al. (1983) studied this question.

synapsesocial.com/papers/6a91a7daeb7f1f9a6abe4852 https://doi.org/10.1093/nar/11.9.2857

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Implication

Biophysical study reveals exothermic groove binding and substantial thermal stabilization of poly d(AT) by netropsin, suggesting that hydrogen bonding and cation release drive complex formation.

Calorimetric and spectroscoptc Investigation of drug - DNA Interactions. I. The binding of netropsin to poly d(AT)

Discussion

Member takes

Implication

Key Points

Cite This Study

Calorimetric and spectroscoptc Investigation of drug - DNA Interactions. I. The binding of netropsin to poly d(AT)

Discussion

Member takes

Implication

Key Points

Cite This Study

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