Surveillance Program of Clinical Samples for Polio and Non-Polio Enteroviruses in the Slovak Republic during the 1958–2020 Period

Figures

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  Figure 1. Temporal trends in probability of enterovirus occurrence in the Slovak Republic from 1958 to 2020. GAM-based estimates (lines) are displayed along with 95% confidence intervals.

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  Figure 2. Temporal trends in probability of the three major enterovirus strains’ (coxsackieviruses (CV) and echoviruses (E)) occurrences in the Slovak Republic from 1958 to 2020. GAM-based estimates (lines) are displayed along with 95% confidence intervals.

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  Figure 3. Canonical correspondence analysis (CCA) ordination plot showing temporal changes in the composition of enterovirus findings in the Slovak Republic from 1958 to 2020. Only the most frequent strains (top 50%) with the best fit (top 50%) to the temporal trend (first axis) are displayed. Ordination scores of these strains are shown in red letters (the position of the label matches the score position) and represent the space where the maxima of relative abundances are expected. Distances between sample scores (dots) represent the similarity of virus assemblage (polioviruses (PVs), coxsackieviruses (CV), and echoviruses (E)) composition among samples. The ordination plot is scaled symmetrically; variation explained by the ordination axes is displayed in parentheses. Note that the sample scores are distinguished by color in decadal steps and the axis values are standardized to zero-weighted means and unit-weighted variances.

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  Figure 4. Relationships between the proportion of all enteroviruses (a), coxsackieviruses B (CVB) (b), and Echoviruses (E) (c) in wastewater versus clinical samples. GLM-based estimates (lines) are displayed along with 95% confidence intervals (gray bands) and observed values (points). Point size is proportional to the number of samples taken in the given year. Note that the position of samples is slightly jittered to avoid overlap. The x axis shows the proportion of enteroviruses in the wastewater sample in percentages, and the y axis show the proportion of enteroviruses in clinical samples.