Role of Asymmetric Dimethylarginine in Inflammatory Reactions by Angiotensin II

Endothelial cells and monocytes (in vitro model)

Incubation with Angiotensin II (10(-6) M) or exogenous ADMA (30 microM) for 24 hours, with or without pretreatment with losartan (1, 3, and 10 muM) or L-arginine

Untreated control cells (implied)

Inflammatory markers including ADMA levels, nitrite/nitrate, ROS, TNF-alpha, IL-8, CXCR2 mRNA expression, endothelial cell adhesion to monocytes, and NF-kappaB activitysurrogate