Lung Transplant Recipients Immunogenicity after Heterologous ChAdOx1 nCoV-19—BNT162b2 mRNA Vaccination

Figures

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  Figure 1. STROBE, STrengthening the Reporting of OBservational studies in Epidemiology.

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  Figure 2. Evolution of serum SARS-CoV-2 IgG titers and neutralizing capacity in vaccinated LTRs, assessed at days 0 (n = 49), 28 (n = 47), 84 (n = 49), 112 (n = 44), 180 (n = 42), 210 (n = 32) and 238 (n = 28). Data are expressed in log10. (a) Quantitative titers of anti-SARS-CoV-2 IgG at consecutive timepoints (geometric mean in BAU/mL ± 95% CI). * p <0.05, ** p <0.01, and *** p <0.001 by the Kruskal–Wallis test followed by a post–hoc Dunn’s tests. (b) Quantitative titers of neutralizing antibodies (NAbs) at consecutive time-points (geometric mean in AU/mL ± 95% CI). *** p <0.001 by the Kruskal–Wallis test followed by a post hoc Dunn’s tests. (c) Evolution of anti-SARS-CoV-2 IgG titers in seropositive LTRs with first dose responders represented in blue (n = 2), second dose responder in green (n = 1), and booster dose responders in orange (n = 7). (d) Evolution of NAbs titers in seropositive LTRs with first dose responders represented in blue (n = 2), second dose responder in green (n = 1), and booster dose responders in orange (n = 7). Patients with discordant results between IgG and NAbs are represented in black (n = 2). Dotted black lines represent the positivity cut-offs for IgG…

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  Figure 3. Evolution of wild-type- and SARS-CoV-2 variant-neutralizing capacities in vaccinated-LTRs. Data are expressed in log10. (a) Dilution titers of anti-SARS-CoV-2 IgG at day 0 (n = 49), 28 (n = 47), 84 (n = 49), 112 (n = 44), 180 (n = 42), 210 (n = 32), and 238 (n = 28), assessed by pseudovirus neutralization test (pVNT) (geometric mean ± 95% CI). Statistical analysis by Kruskal–Wallis test followed by a post hoc Dunn’s test provided non-significant results. (b) Paired Delta and Omicron SARS-CoV-2 neutralizing capacities of wild-type-positive samples (n = 45) assessed by pVNT (geometric mean ± 95% CI). Dotted black lines represent the positivity cut-offs for neutralization (>20 dilution titer, i.e., 1.3 in log10). *** p < 0.001 wild-type vs. Delta and Omicron by the Kruskal–Wallis test followed by a post hoc Dunn’s test. D1 and D2 correspond to the first and second doses of ChAdOx1, respectively; B corresponds to the booster dose, BNT162b2.