What question did this study set out to answer?

To identify and compare the cleavage patterns and roles of ADAMTS and MMP proteases in aggrecan degradation across normal, acutely injured, and osteoarthritic human knee joints.

September 10, 2001Biochemical Journal

Analysis of aggrecan in human knee cartilage and synovial fluid indicates that aggrecanase (ADAMTS) activity is responsible for the catabolic turnover and loss of whole aggrecan whereas other protease activity is required for C-terminal processing in vivo

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Authors

JSJohn D. SandyRush University Medical Center CVChristie VerscharenShriners Hospitals for Children - Tampa

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Implication

Reveals aggrecan fragments from both ADAMTS and MMP cleavage in human joints; leaves open their relative roles in disease.

Key Points

To identify and compare the cleavage patterns and roles of ADAMTS and MMP proteases in aggrecan degradation across normal, acutely injured, and osteoarthritic human knee joints.
Analyzed aggrecan core protein species in vivo from articular cartilage and synovial fluid of normal, acutely injured, and late-stage osteoarthritic human knee joints.
Compared endogenous G1-domain fragments to products generated by in vitro digestion of fetal human aggrecan with MMP-3 and crude aggrecanase.
Synovial fluid from normal joints contained at least 10 aggrecan species, 9 of which were generated by ADAMTS cleavage across all five known aggrecanase sites, with injured joints displaying an increased G1-NITEGE to G1-VDIPEN ratio.
ADAMTS cleavage at the Glu373–Ala374 bond released the bulk of glycosaminoglycan-bearing products into the synovial fluid, whereas MMP-like cleavages occurred in C-terminal regions and retained glycosaminoglycans within cartilage.

Cite This Study

Sandy et al. (2001) studied this question.

synapsesocial.com/papers/6a922efead37002f2338413c https://doi.org/10.1042/bj3580615

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Analysis of aggrecan in human knee cartilage and synovial fluid indicates that aggrecanase (ADAMTS) activity is responsible for the catabolic turnover and loss of whole aggrecan whereas other protease activity is required for C-terminal processing in vivo

Discussion

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Implication

Key Points

Cite This Study

Analysis of aggrecan in human knee cartilage and synovial fluid indicates that aggrecanase (ADAMTS) activity is responsible for the catabolic turnover and loss of whole aggrecan whereas other protease activity is required for C-terminal processing in vivo

Discussion

Member takes

Implication

Key Points

Cite This Study

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