The effects of ethylenediamine tetraacetic acid (EDTA) and related chelating agents on the sensitivity of isolated cell envelopes of some β‐lactamase +ve and ‐ve strains of Gram negative bacteria have been investigated. Envelopes from Pseudomonas aeruginosa (especially strain NCTC 1999) contained the greatest amounts of Mg 2+ and were the most sensitive to these agents in terms of (i) lysis, (ii) release of cations, (iii) release of readily extractable lipid. Cyclohexane—1,2, ‐diamine‐tetraacetic acid was the most effective chelator, followed by EDTA and N ‐hydroxy‐ethylethylenediamine triacetic acid, with nitriloacetic acid and iminodiacetic acid having little effect. A lysozyme–Tris–EDTA system also caused lysis of P. aeruginosa envelopes. The sensitivity of whole cells of the various strains to some β‐lactam antibiotics and other antibacterial agents has been carried out and the basis of sensitivity or resistance in relation to drug destruction and the above envelope composition discussed.
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Haque et al. (1976) studied this question.
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