The triglycerides of sable fish (anaplopoma fimbria). II. fatty acid distribution in triglyceride fractions as determined with pancreatic lipase

Sable fish muscle lipids were fractionated on a silicic acid column with mixtures of chloroform and methanol as eluting solvents. Three main peaks containing only triglycerides were isolated; 11 additional peaks contained phosphorous. Each of the 3 triglyceride peaks was separately fractionated into 300 fractions on silica gel columns impregnated with silver nitrate. Mixtures of petroleum ether and ethyl ether were the eluting solvents. About 25 distinct fractions were isolated from each column. The fractions were characterized for fatty acid content by gas chromatography of the methyl esters. The results showed that the fractionation did not depend upon the presence of single fatty acids but upon total unsaturation. Fatty acid distribution within each fraction was determined with the use of hog pancreatic lipase, followed by thin‐layer chromatography and gas chromatography.