What question did this study set out to answer?

To determine how precentrifugation delay times and varying storage temperatures influence the analytical stability of 48 routine blood analytes.

August 6, 2016Annals of Clinical Biochemistry International Journal of Laboratory Medicine

Effects of time and temperature on 48 routine chemistry, haematology and coagulation analytes in whole blood samples

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To determine how precentrifugation delay times and varying storage temperatures influence the analytical stability of 48 routine blood analytes.
Blood was collected from healthy volunteers into lithium heparin, EDTA, citrate, and sodium fluoride tubes.
Samples were tested immediately or incubated across a range of temperatures (4, 8, 12, 16, 20, 24, 28, or 30°C) for 4, 6, 8, or 24 hours prior to centrifugation and analysis.
Mean percentage deviations were calculated and benchmarked against clinical quality specifications, primarily total allowable error.
Most tested analytes remained within the total allowable error limits, but bicarbonate showed marked instability across virtually all evaluated time points and temperatures.
Potassium, sodium, calcium, and lactate dehydrogenase concentrations were substantially altered by refrigerated temperatures (4°C and 8°C).
Phosphate stability degraded markedly after 8 hours when stored at or above room temperature (≥20°C).

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Implication

Laboratory study demonstrates analyte-specific instability during whole blood storage across temperatures in healthy volunteers, indicating critical boundaries for sample transport.

Cite This Study

Balveren et al. (2016) studied this question.

synapsesocial.com/papers/6a926043e69a445c82384036 https://doi.org/10.1177/0004563216665868

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Implication

Laboratory study demonstrates analyte-specific instability during whole blood storage across temperatures in healthy volunteers, indicating critical boundaries for sample transport.

Effects of time and temperature on 48 routine chemistry, haematology and coagulation analytes in whole blood samples

Key Points

Discussion

Implication

Cite This Study

Effects of time and temperature on 48 routine chemistry, haematology and coagulation analytes in whole blood samples

Key Points

Discussion

Implication

Cite This Study

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