Regulation of glycoprotein D synthesis: does alpha 4, the major regulatory protein of herpes simplex virus 1, regulate late genes both positively and negatively?
Population
Baby hamster kidney (BHK) cell lines (alpha 4/c113 expressing alpha 4 protein, and parental BHK cells)
Comparison
Introduction of the HSV-1 BamHI J fragment vs Comparison between parental BHK cells and alpha…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Highlights dual HSV-1 gD regulation; leaves open alpha 4 repression mechanisms in cell models.
Structured PICO
P
Population
Baby hamster kidney (BHK) cell lines (alpha 4/c113 expressing alpha 4 protein, and parental BHK cells)
I
Intervention
Introduction of the HSV-1 BamHI J fragment (encoding gD, gG, gI, and gE)
C
Comparator
Comparison between parental BHK cells (BJ cell line) and alpha 4-expressing cells (alpha 4/BJ cell line)
O
Outcome
Expression of glycoprotein D (gD) and gEsurrogate
The study demonstrates that the HSV-1 gD gene is subject to both positive and negative regulation, and its lack of expression in alpha 4/BJ cells reflects the expression of the alpha 4 protein.
Cite This Study
Arsenakis et al. (1988) studied this question.