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July 1, 2017IOSR Journal of Applied ChemistryOpen Access

Assessment of Metal - Induced Inhibition of Soybean Urease as a Tool for Measuring Heavy Metals in Aqueous Samples

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Authors

AMA. M. MagomyaJBJ. T. BarminasSOS. A. Osemeahon

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Overview

Laboratory assay reveals concentration-dependent urease inhibition across multiple heavy metals in aqueous solutions, suggesting enzyme-based biosensors can reliably monitor water pollution.

Key Points

  • To evaluate the inhibition of soybean (Glycine max) urease by heavy metal ions as an indirect method for measuring heavy metal concentrations in water.
  • Assayed soybean urease activity across concentrations of Cd2+, Cr3+, As3+, Cu2+, Pb2+, and Zn2+ in both single-metal and multi-metal aqueous solutions.
  • Assessed quantitative assay validity by testing synthetic water samples and comparing the inhibition values against standard atomic absorption spectroscopy (AAS) using a t-test.
  • Concentration-dependent inhibition occurred between 0.001–10 mg/L for Cu2+, As3+, Cr3+, and Cd2+, and 0.1–10 mg/L for Zn2+ and Pb2+, yielding an IC50 potency ranking of Cu2+ > As3+ > Cr3+ > Cd2+ > Zn2+ > Pb2+.
  • Multi-metal solutions showed higher inhibitory strengths than single metals, with calibration plots exhibiting linear ranges of 0.01–10 mg/L for Cu, As, Cr, and Cd, and 0.1–10 mg/L for Zn and Pb.
  • Testing of synthetic water samples demonstrated a 74% agreement between the urease inhibition assay and standard AAS measurements.

Cite This Study

Magomya et al. (2017) studied this question.

synapsesocial.com/papers/6a9275ec6a4f73282e18220dhttps://doi.org/10.9790/5736-1006026170
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