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Spotlight on protein labeling. The labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase (AGT or SNAP-tag) is controlled with both spatial and temporal resolution by cageing the substrate with the 1-(2-nitrophenyl)ethyl group. The substrates can be uncaged simply by illumination. The light-controlled dimerization and light-directed immobilization of SNAP-tag fusion proteins is shown.
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Banala et al. (2007) studied this question.
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