Three sulfonyl benzothiazole-based fluorescent probes (, , and ) for the detection of biothiols (cysteine, homocysteine, and glutathione) are developed based on thiol-mediated nucleophilic aromatic substitutions. The probes exhibited good selectivity and sensitivity toward biothiols over other analytes. The probes were successfully applied for visualizing endogenous thiols in living cells.
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Zhang et al. (2014) studied this question.
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