The employment of gas‐liquid chromatography (GLC) separation factors between methyl esters of unsaturated fatty acids is feasible as a means of tentative identification, either between acids of one chain length and differing numbers of double bonds, or between acids of one chain length and the same number of double bonds in differing positions, provided the acid structures are appropriately grouped by end carbon chain. The modification of separation factors by temperature, chain length, number of double bonds, or position of double bonds is apparent from examination of a larger number of examples than was hitherto available. Examples of the usefulness of separation factors in identifying unknowns or predicting retention times are given.
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R. G. Ackman (1963) studied this question.
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