November 12, 1983Transfusion

The production of platelet controls for assays quantitating platelet‐associated IgG

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Population

Washed 2 percent formalin-fixed platelets

Design

Preclinical

Follow-up

up to 3 months

Authors

GDGregory A. DenommeGrifols (United States)JKJG KeltonUniversité de Tours

Discussion

Member takes

Implication

Provides stable positive controls for PAIgG assays in research; leaves open translation to human clinical samples.

Structured PICO

Population

Washed 2 percent formalin-fixed platelets

Intervention

Incubation with normal serum IgG (Cohn fraction II) for 60 minutes at 37 degrees C, followed by storage at 4 degrees C or -70 degrees C with 60% glycerol

Outcome

Level of platelet-associated IgG (PAIgG) and platelet recoverysurrogate

A reliable method for producing stable positive control platelets for PAIgG assays was developed using formalin fixation and glycerol cryopreservation.

Cite This Study

Denomme et al. (1983) studied this question.

synapsesocial.com/papers/6a928e17cfe2d75514a5fa83 https://doi.org/10.1046/j.1537-2995.1983.23684074275.x

View Full Paper

Ask AI

Bookmark

The production of platelet controls for assays quantitating platelet‐associated IgG

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The production of platelet controls for assays quantitating platelet‐associated IgG

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider