Key result
A simplified micro ELISA procedure measured platelet-associated IgG at 1.7 +/- 0.6 fg/platelet in normal controls, while patients with ATP had values two to seven times higher.
The described micro ELISA procedure provides a simplified method for measuring platelet-associated IgG, demonstrating elevated levels in patients with ATP compared to normal controls.
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Should not yet change ATP evaluation; leaves open diagnostic utility of micro ELISA pending validation.
A simplified micro ELISA procedure to measure platelet-associated IgG is described. The platelet-bound IgG first is extracted into the fluid phase by solubilizing washed platelets in 0.1% triton X-100. The solubilized IgG in the extract and IgG standards are incubated in microtiter wells previously coated with antihuman IgG. The IgG in the standards and extract bind to the solid phase antihuman IgG. The bound IgG then is measured by the addition of peroxidase labeled antihuman IgG and appropriate substrate. With this method platelets from normal controls were found to have 1.7 +/- 0.6 fg IgG/platelet (mean +/- SD). Platelets from patients with ATP had values that were two to seven times the control values. The relative advantage of this technic is discussed.
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Yesus et al. (1984) studied Autoimmune thrombocytopenic purpura (ATP). Micro ELISA procedure for PAIgG measurement vs. Normal controls was evaluated on Platelet-associated IgG (PAIgG) levels (two to seven times the control values). A simplified micro ELISA procedure measured platelet-associated IgG at 1.7 +/- 0.6 fg/platelet in normal controls, while patients with ATP had values two to seven times higher.
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