January 1, 2010Thrombosis and Haemostasis

Development of a high-throughput ELISA assay for platelet function testing using platelet-rich plasma or whole blood

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Population

15 healthy donors

Design

Preclinical

Authors

KBKatleen BroosResearch Institute for Chromatography AFAlexandre FontayneHôpital de la Croix-Rousse TSTímea SzántóUniversity of Helsinki

Discussion

Member takes

Implication

Should not yet change platelet testing practice; leaves open clinical utility pending validation in larger cohorts.

Structured PICO

Population

15 healthy donors

Intervention

High-throughput sandwich ELISA assay for platelet function testing using monoclonal antibodies against P-selectin or alphaIIbbeta3 and GPIbalpha in the presence of five different agonists (ADP, TRAP, U46619, collagen-related-peptide, and arachidonic acid)

Outcome

Platelet response to agonists (binding of platelets to antibodies)surrogate

A newly developed high-throughput ELISA assay can reliably evaluate multiple platelet signaling pathways using minimal volumes of platelet-rich plasma or whole blood.

Cite This Study

Broos et al. (2010) studied this question.

synapsesocial.com/papers/6a72019731a3df824329ea2c https://doi.org/10.1160/th09-07-0505

Topics

Dual antiplatelet therapy

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Development of a high-throughput ELISA assay for platelet function testing using platelet-rich plasma or whole blood

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Topics

Development of a high-throughput ELISA assay for platelet function testing using platelet-rich plasma or whole blood

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Topics

Also Consider