What question did this study set out to answer?

To elucidate the functional role of the ribosomal biogenesis factor GhBOP1 in upland cotton (Gossypium hirsutum) leaf morphogenesis.

September 1, 2022International Journal of Molecular SciencesOpen Access

GhBOP1 as a Key Factor of Ribosomal Biogenesis: Development of Wrinkled Leaves in Upland Cotton

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Authors

YWYanwen WangZhejiang University of Science and Technology ZSZhimao SunShaanxi Normal University LWLong WangJiangnan University

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Implication

Experimental gene silencing reveals that GhBOP1 loss causes wrinkled leaves in upland cotton, highlighting its role in ribosome biogenesis and hormone signaling.

Key Points

To elucidate the functional role of the ribosomal biogenesis factor GhBOP1 in upland cotton (Gossypium hirsutum) leaf morphogenesis.
Characterized protein interactions and nucleolar localization of GhBOP1, GhPES, and GhWDR12 using yeast two-hybrid, bimolecular fluorescence complementation, and luciferase complementation assays.
Suppressed GhBOP1 expression in upland cotton using virus-induced gene silencing (VIGS) and analyzed leaf anatomy via paraffin sectioning.
Profiled expression changes in cell cycle progression, jasmonic acid pathway markers (GhOPR3), and auxin-related genes.
GhBOP1 localized to the nucleolus and physically interacted with GhPES and GhWDR12.
Silencing GhBOP1 led to uneven leaf margin bending and young wrinkled leaves displaying disorganized palisade tissue and enlarged upper epidermal cells.
GhBOP1 suppression impaired cell cycle progression, upregulated the jasmonic acid-related gene GhOPR3, and downregulated auxin-related genes.

Cite This Study

Wang et al. (2022) studied this question.

synapsesocial.com/papers/6a92913e55c87725f749fd6d https://doi.org/10.3390/ijms23179942

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Implication

Experimental gene silencing reveals that GhBOP1 loss causes wrinkled leaves in upland cotton, highlighting its role in ribosome biogenesis and hormone signaling.

GhBOP1 as a Key Factor of Ribosomal Biogenesis: Development of Wrinkled Leaves in Upland Cotton

Discussion

Member takes

Implication

Key Points

Cite This Study

GhBOP1 as a Key Factor of Ribosomal Biogenesis: Development of Wrinkled Leaves in Upland Cotton

Discussion

Member takes

Implication

Key Points

Cite This Study

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