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A column chromatography method for separation of phenolic compounds from flaxseed using Sephadex LH‐20, RP‐8 and silica gel is described. TLC analysis proved that separated fractions contained numerous phenolic compounds. All fractions exhibited a maximum UV absorption between 270 and 290 nm. Additional shoulders were observed between 274 and 350 nm. These results imply that lignans and their derivatives are the main phenolic compounds present in flaxseed.
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Amarowicz et al. (1994) studied this question.
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