What question did this study set out to answer?

Establish and validate a high-throughput phenotypic in vitro platform using spontaneously active primary dorsal root ganglion (DRG) cultures on multiwell multielectrode arrays (MEAs) for screening analgesic compounds.

April 14, 2016Journal of NeurophysiologyOpen Access

Development of a spontaneously active dorsal root ganglia assay using multiwell multielectrode arrays

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Authors

KNKim M. NewberryCommonwealth Scientific and Industrial Research Organisation SWShuya WangSultan Idris Education University NHNina HoqueBristol-Myers Squibb (United States)

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Implication

In vitro study demonstrates pharmacological modulation of spontaneous firing in rat dorsal root ganglion neurons, indicating high-throughput utility for screening pain therapeutics.

Key Points

Establish and validate a high-throughput phenotypic in vitro platform using spontaneously active primary dorsal root ganglion (DRG) cultures on multiwell multielectrode arrays (MEAs) for screening analgesic compounds.
Plated primary rat DRG cells onto multiwell MEA platforms to record network-level spontaneous electrophysiological firing.
Profiled the assay using selective ion channel blockers (Na+, Ca2+, and K+ channels), pain mediators (capsaicin and bradykinin), and neurokinin receptor antagonists.
Validated the assay's translational relevance by treating cultures with clinically used neuropathic pain therapeutics.
Spontaneous DRG firing was attenuated by selective Na+ and Ca2+ channel blockers and increased by K+ channel blockers.
Neuronal activity was augmented by the TRPV1 agonist capsaicin and the peptide bradykinin, and completely blocked by neurokinin receptor antagonists.
Clinically established neuropathic pain medications suppressed spontaneous DRG firing, confirming the utility of the assay for phenotypic analgesic screening.

Cite This Study

Newberry et al. (2016) studied this question.

synapsesocial.com/papers/6a9296586a4f73282e183308 https://doi.org/10.1152/jn.01122.2015

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Implication

In vitro study demonstrates pharmacological modulation of spontaneous firing in rat dorsal root ganglion neurons, indicating high-throughput utility for screening pain therapeutics.

Development of a spontaneously active dorsal root ganglia assay using multiwell multielectrode arrays

Discussion

Member takes

Implication

Key Points

Cite This Study

Development of a spontaneously active dorsal root ganglia assay using multiwell multielectrode arrays

Discussion

Member takes

Implication

Key Points

Cite This Study

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