A rapid procedure for determining phenols in olive oils, based on an organic-phase enzyme electrode, is described. Direct assays are performed in chloroform solutions which support the tyrosinase activity. This class (phenol)-specific enzyme strongly adheres to the surface of the graphite transducer. The resulting wall-jet detector offers effective flow injection operation, with a detection limit of 4×10−7 M (0.8 ng) phenol and sample frequency of 60 h−1. Applicability to olive-oil samples of different origins is illustrated.
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Wang et al. (1992) studied this question.
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