Key result
Concanavalin A- and thrombin-induced release of serotonin in rat platelets are markedly dependent upon extracellular calcium, with Con A not producing aggregation in calcium-supplemented medium.
Concanavalin A, in combination with creatine phosphate and creatine phosphokinase, provides a valuable method for analyzing calcium-dependent release in platelets without inducing aggregation.
Answers are grounded in this paper and cite their sources.
Rat platelet findings require human validation before any clinical use; extends in vitro tools for calcium-dependent release studies without aggregation.
Effects of the platelet secretagogues thrombin and Concanavalin A (Con A) on shape change and release have been studied under conditions where external Ca2+ and ADP have been controlled. 2. In the absence of detectable aggregation both Con A- and thrombin-induced release of serotonin are markedly dependent upon extracellular calcium. 3. Con A, unlike thrombin, does not produce aggregation in calcium-supplemented medium. 4. The above property of Con A suggests that, in combination with added creatine phosphate and creatine phosphokinase, this ligand will be of value in the analysis of calcium-dependent release in platelets.
No takes yet. Share an insight, caveat, or question.
Belville et al. (1979) studied this question. Thrombin and Concanavalin A was evaluated on Shape change, aggregation, and serotonin release. Concanavalin A- and thrombin-induced release of serotonin in rat platelets are markedly dependent upon extracellular calcium, with Con A not producing aggregation in calcium-supplemented medium.
Synapse has enriched 10 related papers. Consider them for comparative context: